Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 25;11:565134. doi: 10.3389/fpls.2020.565134

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Funck, Baumgarten, Stift, von Wirén and Schönemann

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Subcellular localization of P5CS1 and P5CS2. GFP and chlorophyll fluorescence images of mesophyll protoplasts isolated from wildtype and P5CS1:GFP or P5CS2:GFP expressing plants. Spectrally resolved confocal fluorescence images with 20 channels spanning 490 to 668 nm emission wavelength were used for spectral unmixing with GFP and chlorophyll reference spectra. The GFP images and the overlay demonstrate that fluorescence emission from chloroplasts was entirely attributable to chlorophyll, and GFP fluorescence was exclusively detected in the cytosol. Compare with the simulated channel splitting mode images in Supplementary Figure S1 .