Figure 4.

Influence of proline and NaCl on root growth of p5cs1 and p5cs2 mutants. Seedlings were pre-cultivated for 4 days on plates with half-strength MS medium supplemented with 15 mM sucrose (Suc) and 2 mM proline (Pro) to support establishment of p5cs2 mutant seedlings. Then, established seedlings were transferred to vertical plates containing half-strength MS medium with 15 mM Suc without supplements (A), or supplemented with 2 mM Pro (B), 150 mM NaCl (C), or both (D). Arrows indicate time point of seedling transfer, after which root length was monitored for further 15 days. Diamonds represent the mean ± SD of 5 to 10 seedlings per genotype that developed continuously until day 19. The response of p5cs2 mutants to the individual or combined treatments was significantly different from that of wildtype plants and p5cs1-4 mutants ( Supplementary Table S2 ).