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. 2020 Sep 25;8:580899. doi: 10.3389/fchem.2020.580899

Table 1.

Spectroscopic properties of diboronate and monoboronate molecular probes for detection of biological oxidants.

No. Name Probe Oxidation product Comment References
λabs (nm) ε (M−1cm−1) λem (nm); ϕ λexc (nm) ε (M−1cm−1) λem (nm); ϕ
DIBORONATE PROBES
3 PF1 NA - NA 450a - ca. 515a Chang et al., 2004
4 CPF1 - - - 450 - 520 Purdey et al., 2015
5 EEPF1 - - - 450 - 520 Purdey et al., 2015
6 NPF1 345i 22 400i - 598i - 660i Albers et al., 2008
7 PX1 350a 4 700a 400(weak)a 350a - ca. 450a Miller et al., 2005
8 PR1 NA - NA 530a - ca. 580a Miller et al., 2005
Weber et al., 2018
9 Pinkment - - - 545b - ca. 580 Odyniec et al., 2018
10 Pinkment-OAC - - - 545b - ca. 580 Odyniec et al., 2018
11 azaBDPBA 655 - 682; 0.6 - - 724j Liu et al., 2015
12 TPE-BO - - - ca. 400 - ca. 500 AIE-fluorescence Zhang et al., 2015
13 TPE-DABA - - - 380b ca. 500b AIE-fluorescence Liu et al., 2016
14 TPE-DABF ca. 270
ca. 350		 - - 430b 576b AIE-fluorescence Liu et al., 2016
MONOBORONATE PROBES
15 PC1 480a 4800a 584a; 0.006a 550a - - k(H2O2) ≈ 1 M−1s−1a
k(HOCl) = 1.1 × 104 M−1s−1b
k(ONOO−) = 1 × 106 M−1s−1b 		Miller et al., 2007
Debowska et al., 2016
16 PG1 460a 5500a 510a; 0.075a 450a - - k(H2O2) ≈ 1.1 M−1s−1a Miller et al., 2007
17 PF2 n/a n/a n/a 475a 28 600a 511; 0.27a k(H2O2) ≈ 0.47 M−1s−1a Dickinson et al., 2010
18 PF3, Fl-B 454a 24 000a 521; 0.1a 492a 88 000a 515; 0.94a k(H2O2) ≈ 0.38 M−1s−1a
k(H2O2) = 0.65 M−1s−1b
k(HOCl) = 4.3 × 102 M−1s−1b
k(ONOO−) = 1.1 × 106 M−1s−1b 		Dickinson et al., 2010
Rios et al., 2016
19 PE1 480a 16 400a 519; 0.3a 491a 51 200a 514; 0.93a k(H2O2) ≈ 0.81 M−1s−1a Dickinson et al., 2010
20 PY1 494a 16 200a 558; 0.1a 519a 48 900a 548; 0.12a k(H2O2) ≈ 0.37 M−1s−1a Dickinson et al., 2010
21 PO1 507a 13 900a 574; 0.07a 540a 29 300a 565; 0.46a k(H2O2) ≈ 0.52 M−1s−1a Dickinson et al., 2010
22 FlAmBE - - - 485b - 535b k(ONOO−) > 5 × 105 M−1s−1 Zielonka et al., 2012b
Zielonka et al., 2012a
23 NucPE1 468a
490a 		27 300a
26 000a 		530; 0.117a 505a 19 100a 530; 0.626a k(H2O2) ≈ 0.82 M−1s−1a Dickinson et al., 2011b
24 GSH-PF3 - - - 488 - 512 ONOO− and GSH detection Sedgwick et al., 2018
25 CBE - - - 332 - 450 - Du et al., 2008
26 CBA 287b 12 000b - 324b 13 000b 450b k(H2O2) = 1.5 M−1s−1b;
k(ONOO−) = 1.1 × 106 M−1s−1b 		Zielonka et al., 2010
Zielonka et al., 2012b
27 p-NPBA 294c - - 405c 19 400c - Colorimetric probe Lu et al., 2011
28 OTB - - - 366 - 466 The organic thin-film fluorescence probe for sensing of hydrogen peroxide vapor Fu et al., 2016
29 OTFB - - - - - - The organic thin-film fluorescence probe for sensing of hydrogen peroxide vapor Fu et al., 2016
30 OTPB - - - - - - The organic thin-film fluorescence probe for sensing of hydrogen peroxide vapor Fu et al., 2016
31 PyBor 265b
275b
325b
340b 		41 550b
69 970b
44 120b
59 450b 		377, 392, 417; 0.08b 268b
280b
350b
360b
385b 		55 160b
95 610b
65 280b
29 350b
25 190b 		396, 410, 430; 0.60b Yu et al., 2012
32 BN 344d - 455d 455d - 555d Probe designed for ACh detection Liu et al., 2014
33 - 376e - 536; 0.54e 424e - 604, 0.18e Ratiometric probe Lee et al., 2019
34 DSTBPin ca. 355f - 488f 350f - 444f Increase in fl. intensity Lampard et al., 2018
35 MSTBPin ca. 330f - 405f 330f - 380f Increase in fl. intensity Lampard et al., 2018
36 STBPin ca. 320f - ca.360f 315f - ca. 360f Small decrease in fl. Intensity Lampard et al., 2018
37 CSTBPin ca. 330f - ca. 380f 330f - ca. 380f Decrease in fl. Intensity Lampard et al., 2018
38 NDSTBPin 360f - 525f 350f - ca. 510f Decrease in fl. Intensity Lampard et al., 2018
39 DAPOX-Bpin 350f - 480f 350f - 475f Increase in fl. intensity Lampard et al., 2018
40 TPR1 226b 6700b 545; 0.029b 280b - 545; 0.054b Terbium-based fluorogenic probe Lippert et al., 2010
41 463 36 500 565 482 - 640 Ratiometric I640/I565; probe designed for HOCl detection Tong et al., 2018
42 DCM-Bpin 434 - - 560 - 667 Wu L. et al., 2019
43 TCF-OCl, TCFB2 400 - 590 560 - 606 Probe designed for HOCl detection Shu et al., 2015
Sedgwick et al., 2017
Choudhury et al., 2019
44 BSD 391g - - 522g 87 000g - Colorimetric probe Zhan et al., 2010
45 AzuFluor 483-Bpin 327f - - 335f - 483 Murfin et al., 2019
46 478 61 300 - ca. 480 >61 300 522 kobs(ONOO−) = 4.2 × 103 M−1s−1
Formation of the fluorophore via intramolecular cyclization of oxidation product		 Zhang J. et al., 2016
47 BTCBE 356h - - 441h - 522; 0.088h Shu et al., 2020a
a

20 mM HEPES buffer, pH 7, 25°C.

b

Phosphate buffer, pH 7.4, 25°C.

c

75 mM carbonate/bicarbonate buffer, pH 9.0.

d

Phosphate buffer, pH 7.5.

e

10 mM phosphate buffer, pH 7.4, containing 50% (v/v) DMSO.

f

H2O/CH3OH (52.1 wt%), pH 8.2.

g

NaHCO3/Na2CO3 buffer, pH 9.

h

PBS/ethanol = 7:3, pH 7.4.

i

20 mM HEPES buffer, pH 7.5.

j

PBS/ethanol 1:1, pH 7.4.