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. 2020 Sep 25;8:580899. doi: 10.3389/fchem.2020.580899

Table 3.

Mitochondria-targeted boronate-based molecular probes for detection of biological oxidants.

No. Name Probe Oxidation product Comment References
λabs (nm) ε (M−1cm−1) λem (nm); ϕ λexc (nm) ε (M−1cm−1) λem (nm); ϕ
74 MitoPY1 489a
510a 		14,300a
14,200a 		540; 0.019a 510a 22,300 528, 0.405a k(H2O2) ≈ 0.201 M−1s−1 Dickinson and Chang, 2008
75 MitoB, m-MitoPhB(OH)2 - - - HPLC detection: λAbs = 268 nm or LC-MS detectionr;
k(H2O2) ≈ 3.8 M−1s−1b
k(ONOO−) = 1 ×106 M−1s−1 		Cochemé et al., 2011
Sikora et al., 2013
76 o-MitoPhB(OH)2 - - - HPLC detection: λAbs = 268 nm or LC-MS detectionr;
k(ONOO−) = 3.5 × 105 M−1s−1 		Sikora et al., 2013
77 p-MitoPhB(OH)2 - - - HPLC detection: λAbs = 268 nm or LC-MS detection;
k(ONOO−) = 1 × 106 M−1s−1 		Sikora et al., 2013
78 SHP-Mito 342d 17,000d 470; 0.13d 383d 15,000d 545; 0.12d Ratiometric probe;
Two-photon probe;
k(H2O2) ≈ 1.0–1.2 M−1s−1d 		Masanta et al., 2012
79 Mito-H2O2 490e 527; 0.003e 376e 527e Increase in fl. intensity Xu J. et al., 2016
80 MI-H2O2 425f 0.0087f 525f 555; 0.11f k(H2O2) ≈ 4.35 M−1s−1f Xiao et al., 2016
81 CBZ-H2O2 340g ca. 400g 350g 430g Zhang K. et al., 2016
82 507h 0.002h 495h 515; 0.213h Probe designed for HOCl detection Li G. et al., 2017
83 HKB 564i 666i 440i 594i Ratiometric probe;
k(H2O2) ≈ 1.07 M−1s−1i 		He et al., 2018
84 HBTPB 312j 539j 373j 669j Ratiometric probe; ESIPT-based probe Tang et al., 2018
85 500k 640k 500k 535k Ratiometric probe Shen et al., 2018
86 pep3-NP1 352
455l 		9020
10,700l 		646; 0.033l 455l 555; 0.058l k(H2O2) ≈ 0.487 M−1s−1l;
DNA-binding probe		 Wen et al., 2015
87 Mito-HP 358m 543m 446m 543m Increase in fl. intensity Dai et al., 2018
88 Mito-NP 360n 460n 553n ICT-based probe;
k(H2O2) ≈ 3.5 M−1s−1 		Liang et al., 2019
89 MBTBE 380o 0.018o 520o 569; 0.097o Shu et al., 2020b
90 CM 400p 340p ca. 450p Ratiometric probe Weber et al., 2020
91 Mito-HT 380q 493; 0.39q 395q 562; 0.43q Ratiometric probe Wang C. et al., 2020
a

20 mM HEPES, pH 7, 25°C.

b

KCl medium, pH 8.0, 25°C.

c

50 mM phosphate buffer, pH 7.4, 1 M CH3OH, RT.

d

30 mM MOPS, 100 mM KCl, pH 7.4.

e

20 mM phosphate buffer, pH 7.4, 1% DMSO, 25°C.

f

10 mM PBS, pH 8.0.

g

20 mM PBS, pH 7.4, 20% DMSO.

h

20 mM PBS, pH 7.4, 1% DMSO.

i

10 mM PBS, pH 7.0, 30% CH3CN.

j

10 mM HEPES/CH3CN (1/1, v/v), pH 7.4.

k

PBS/CH3CN, (1/9, v/v), pH 7.4.

l

Tris-HCl, pH 7.2, 1% DMSO.

m

10 mM PBS, pH 7.4, RT.

n

20 mM PBS, pH 7.4.

o

PBS : C2H5OH, 7:3, pH 7.4.

p

PBS/CH3OH (52%), pH 7.4.

q

0.01 M PBS/DMSO (5/1, v/v), pH 7.4.

r

Molecular ions used in MS/MS-based detection are listed in Supplementary Table 1.