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. Author manuscript; available in PMC: 2020 Oct 9.
Published in final edited form as: Neurobiol Dis. 2020 Jan 3;136:104725. doi: 10.1016/j.nbd.2019.104725

Fig. 4.

Fig. 4.

A protective dose of adaptaquin block the induction of ATF4, CHOP and Trib3 proteins in neuronal PC12 cells treated with 6-OHDA and maintains Parkin protein levels. Western blot images (A) and corresponding quantifications (B–E) of 5 independent experiments showing the protein levels of ATF4 (B), CHOP (C), Trib3 (D) and Parkin (E) in neuronal PC12 cells treated with DMSO, 0.1 μM or 0.5 μM AQ and 150 μM 6-OHDA for 8 h. In DMSO-treated cultures, 6-OHDA induces ATF4, CHOP and Trib3 proteins and decreases Parkin protein (ANOVA with Tukey’s post-hoc tests, #p ≤ .05). By contrast, cultures treated with 6-OHDA and 0.5 μM displayed a significant decrease in Trib3, ATF4 and CHOP protein levels (ANOVA with Tukey’s post-hoc tests, *p < .05, **p < .001). In addition, a 0.5 μM dose of AQ significantly increased Parkin levels (ANOVA with Tukey’s post-hoc tests, *p = .05). Data are normalized to ERK. Non specific bands are marked with asterisks. Survival assay (F) showing the percentage of remaining dopaminergic neurons in ventral midbrain cultures infected with a lentivirus carrying a control shRNA (shControl) or an shRNA against Parkin (shParkin) for 14 days and immunostained for TH and GFP. Parkin knockdown induces the death of 31% of TH/GFP+ neurons (t-test, ***p < .001).