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. 2020 Sep 18;10(25):11549–11561. doi: 10.7150/thno.44251

Figure 4.

Figure 4

The effect of CXCR4 on autophagy. (A) AGS-EBV cells exposed to siCXCR4 for 48 h to detect LC3B-I/II expression. Data are expressed as the mean ± SD. *p<0.05. (B) Immunofluorescence staining was performed to detect autophagy in AGS-EBV cells treated with siCXCR4 and control. (C) Electron micrographs of autophagosome changes in the control (a), siCXCR4-1 (b) and siCXCR4-2 (c) groups. (D) Differential expression of the identified autophagy-related genes was confirmed by qRT-PCR in AGS-EBV cells transfected with siCXCR4 or control. (E) The expression of CXCR4 and its downstream autophagy genes was detected in cells transfected with siCXCR4 and in control cells. (F) Increased ATG7 expression level was observed after ZEB1 overexpression. (G) Structure of the ATG7 promoter reporter plasmid. The bottom panel is the consensus binding sequence motif of ZEB1. (H) Promoter-driven luciferase activity of wild-type and mutant ATG7 in 293T cells. Cells were transfected with ZEB1 or control plasmid. (I) ZEB1 activated ATG7 by binding to the ATG7 promoter, which was detected using a dual luciferase reporter system.