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. 2020 Sep 15;10(25):11428–11443. doi: 10.7150/thno.47432

Figure 3.

Figure 3

Up-regulation of SPP1 predicts poor prognosis in human melanoma. (A-B) Relative expression levels of SPP1 in HEK-293T, HaCat cells, melanoma cell lines, and patients-derived melanoma short-term cultures (STCs) quantified by RT-PCR (A) and western blotting (B). (C-D) Quantification by RNA-seq of SPP1 expression between melanoma and melanoma-adjacent tissue (N (nontumor) = 11; N (tumor) = 19) (C), between melanoma and paired melanoma-adjacent tissue from Xiangya melanoma cohort (N = 11) (D). (E-F) The representative protein expression of SPP1 (E) and percent distribution (F) between normal skin and melanoma tissues in The Human Protein Atlas (https://www.proteinatlas.org). (G) SPP1 expression grouped by pathologic grades of melanoma from GEPIA. (H) Kaplan-Meier survival analysis of high and low SPP1 expression groups in Xiangya melanoma cohort. Error bars represent SD. *, P < 0.05; **, P < 0.01.