Figure 1.
Aβ1-40-injected mice exhibit AMD-like pathologies. (A) Fundus photography of the eyes of PBS- and Aβ1-40-injected WT mice. Multiple patchy deposits (black arrows) were found in the Aβ1-40-treated group at 2 and 4 days following the injection. Scale bar = 500 µm. (B) Representative images of retinal sections of the Aβ1-40-treated group within 200 µm from the optic disc on days 0, 2, and 4. Scale bar = 100 µm. (C) Changes in the thickness of the INL, ONL, and total retina and the ratio of ONL/total retina thickness. The ONL thickness was significantly increased after 2 days of Aβ1-40 deposition and significantly decreased after 4 days compared with the PBS-treated controls. GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; ONL: outer nuclear layer; IS: inner segment; OS: outer segment; RPE: retinal pigment epithelium. (D) Average traces from ERG recordings and (E) maximum scotopic ERG a- and b-wave amplitudes from mice at 0, 2, and 4 days post-injection. All results are presented as the mean ± SEM; n = 4-5 for each group. NS = nonsignificant, *P < 0.05. **P < 0.01, ***P < 0.001, Student's t-test, SEM: standard error of the mean.