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. 2020 Sep 19;10(25):11622–11636. doi: 10.7150/thno.47533

Figure 1.

Figure 1

Rationally designed BCL10 peptide inhibitors (BPIs) inhibited BCL10 filament formation. A. BCL10 peptide inhibitor candidates were designed according to the BCL10 structure model (PDB ID: 6BZE). The sequences of BPIs are shown in the same colours as their counterparts. BCL10-Ctl consists of only the membrane translocation (MT) portion. B. Negative-stained EM micrographs showed the effect of different BPI candidates on BCL10 filament formation. BCL10-P2 and BCL10-P4 effectively inhibited BCL10 filament formation. C. BCL10-P2 and BCL10-P4 coimmunoprecipitated with BCL10. D-E. HeLa cells were transfected with 1 µg of pcDNA4-myc-Bcl10 vector and, after 24 h, were treated with the indicated BPIs (100 µM) for 24 h. Representative immunofluorescence images show the effect of BPIs on BCL10 filament formation in HeLa cells. E. Cells containing BCL10 filaments were counted in over 10 HPF. The percentage of cells containing BCL10 filaments was calculated and is shown in E. The corresponding statistics were calculated. The data are reported as the mean ± SD of three independent experiments. Statistics: one-way ANOVA with Dunnett's multiple comparison test, *p < 0.05.