Figure 2.
Evidence of eNOSactivation in CMECs through a cardiomyocyte-dependent paracrine pathway. A) Schematic of the experimental protocol for investigating the effects of conditioned medium of CMs or Fbson CMECs. B-C) Dead CMECs in flow cytometry assay (n=3). D-E) The p-eNOSand t-eNOSwere detected by Western Blot (n=4). *P<0.05 vs. Control. CM: cardiomyocyte; CMTXL: TXL-pretreated cardiomyocyte; Fb: fibroblast; FbTXL: TXL-pretreated fibroblast; Reox: reoxygenation. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.