Figure 4.

The improvement of cardiac microcirculation and infarct-sparing effect induced by TXL is mediated by sEVs.A-B) Representative perfusion images for myocardial microcirculatory perfusion (percentage relative to baseline) measured by laser Doppler flow during myocardial I/R (n=6-8), and the statistical graph were shown, respectively. C) Representative images for myocardial infraction size assessed by Evans blue/TTC double staining (n=7-8). D-E) Quantitative analysis for infarct size and area at risk after I/R, respectively. F) Serum CK-MB activity in each group (n=5-6). G-H) The p-eNOSand t-eNOSwere detected by Western Blot (n=4). * P<0.05 TXL vs. Control; # P <0.05 TXL + GW4869 vs TXL. AAR: area at risk; IA: infarct area; TA: total area. All data are mean ±SEM. Statistical analysis was performed with two-way (microcirculatory perfusion) or one-way (other experiments) ANOVA followed by Tukey's test.