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. 2020 Sep 26;10(25):11820–11836. doi: 10.7150/thno.48297

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Effects of PNE on DNA methylation of AT₁R and AT₂R promoter in male offspring. Left ventricle tissues were freshly isolated from male offspring that had been perinatally exposed to saline and nicotine. DNA sample isolated and methylation levels were determined by methylation specific PCR analysis. (A) Methylation levels in AT₁R promoter region. (B) Methylation levels in AT₂R promoter region. Data are means ± SEM (n=4~5 animals/group). *P < 0.05 vs. control, #P <0.05 vs. without LNA-miR-181a treatment, as determined by two-way ANOVA test.