Figure 7.

Effects of PNE on autophagy protein expression in male offspring. Left ventricle tissues were freshly isolated from male offspring that had been perinatally exposed to saline and nicotine. The protein levels of autophagy-related genes were determined by Western blot analysis and were normalized as fold of GAPDH density. (A) Atg-5 protein density in the absence of LNA-miR-181a, (B) Atg-5 protein density in the presence of LNA-miR-181a, (C) Beclin-1 protein density of each group, (D) p62 protein density of each group, (E) LC3B-I and LC3B-II protein density in the absence of LNA-miR-181a, (F) LC3B-I and LC3B-II protein density in the presence of LNA-miR-181a. Data are means ± SEM (n=4~5 animals/groups). *P < 0.05 vs. control, as determined by Student's t-test.