Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 25;8:559554. doi: 10.3389/fcell.2020.559554

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Teixeira, Vijaya Kumar, Kumar Katakam, Cocola, Pelucchi, Graf, Kiesel, Reinbold, Pavão, Greve and Götte.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Functional assays for the CSC-phenotype. MDA-MB-231 and MCF-7 cell lines modified to overexpress HS2ST1 and HS3ST2 and control cells were submitted to a series of functional assays to investigate the acquisition of a CSC-phenotype. From top to bottom: (A, Colony Formation) Percentage of colonies relative to control (100%) present in each well after 14 days. (B, Spheroid formation) Examples of spheres found in the drops (left). Area of the spheres relative to the vector controls (100%) present in each drop after 1 week (right). (C, Mammosphere assay) Examples of mammospheres generated after 15 days (top). Percentage of mammospheres formed per number of plated cells relative to control (100%) in the MCF-7 cell line after 9 or 15 days. Data represent mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.