FIGURE 1.

Growth-promoting characteristics of strain YC0136. (A) Detection of IAA secretion. A single clone of strain YC0136 was inoculated in Landy liquid medium and cultured for 4 days at 25°C, 90 rpm. Salkowski solution was then used to detect IAA secretion. A standard solution with 50 mg/L IAA was used as a positive control. Landy medium without inoculation was used as a negative control. (B) Qualitative analysis of phosphate solubilization. A single clone of strain YC0136 was cultivated on LB medium at 37°C. The pure bacterial culture was inoculated onto organophosphorus culture medium for qualitative analysis of phosphate solubilization. (C) Strain YC0136 inhibits the growth of R. solanacearum. R. solanacearum was coated on LB ager medium, and then strain YC0136 was inoculated onto the coated plate and cultured for 24 h at 37°C. (D) Strain YC0136 inhibits the growth of F. moniliforme. Hyphae of F. moniliforme were placed in the center of PDA medium and cultured for 2 days at 28°C. Strain YC0136 was then streaked around the fungi and cultured at 28°C for another 2 days.