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. 2020 Sep 25;11:584174. doi: 10.3389/fmicb.2020.584174

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Copyright © 2020 Liu, Wang, Sun, Han, Peng, Liu, Liu, Ding, Wang and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-qPCR assay of genes in tobacco and YC0136. Fluorescence RT-qPCR to verify the accuracy of the transcriptome data. One microgram of purified total RNA was used as template for first-strand cDNA synthesis. Several genes from RNA-seq were selected for amplification by SYBR green RT-qPCR. The GAPDH gene of strain YC0136 and the actin gene of tobacco were selected as references. Relative expression levels were calculated using the delta-delta Ct method. (A) RT-qPCR results for genes related to plant hormone transduction in tobacco. (B) RT-qPCR results for genes related to phenylpropane compound metabolism in tobacco. (C) RT-qPCR results for genes in strain YC0136. Three biological replicates were carried out, and three technical replicates were performed. Error bars indicate the SD from the mean.