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. 2020 Sep 25;10:429. doi: 10.3389/fcimb.2020.00429

Figure 4.

Figure 4

(A) T. gondii-triggered NETs release degraded by DNase I. PMNs were pretreated with DNase I (90 U) or DNase I (180 U) for 15 min, and then incubated with viable T. gondii tachyzoites (ratio 1:1) for 90 min. NETs release triggered by T. gondii was quantified with Pico Green and the fluorometric reader. Data were expressed as mean ± SD (n = 3). (B) Increased ROS levels in T. gondii tachyzoites-triggered NETs release. PMNs were incubated with viable T. gondii tachyzoites (ratio 1:1 or 1:2) for 90 min. NETs release triggered by T. gondii was tested by the fluorometric reader. Data were expressed as mean ± SD (n = 3). P < 0.05 were considered significant (**P < 0.01 and ***P < 0.001).