FIGURE 1.

NP from the IAV interacts with host FLNA protein conservatively. (A) HEK293 cells were transfected with either 5 μg of pcDNA3.1, NP(WSN), NP(HK) or NP(PR8) plasmids (n = 3) (see Supplementary Figures 1–3). 24 h post-transfection, cells were harvested. IP was setup using mouse anti-FLNA antibody and NP was detected in the eluate by Western blotting using rabbit anti-NP antibody. (B) A549 cells were infected with IAV PR8 (MOI = 1). 24 h post-infection, cells were harvested. IP was setup using mouse anti-NP antibody and FLNA was detected in the eluate by Western blotting using rabbit anti-FLNA antibody. (C) A549 cells were infected with IAV PR8 (MOI = 5) and HKX31 (MOI = 1) (n = 3). IP was setup using mouse anti-FLNA antibody and anti-IgG mouse antibody and NP was detected in the anti-FLNA antibody IP eluate by Western blotting using mouse anti-NP antibody (see Supplementary Figure 16).