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. 2020 Sep 25;11:581867. doi: 10.3389/fmicb.2020.581867

FIGURE 10.

FIGURE 10

Reduced cell survival was observed post-NP transfection and FLNA silencing in an IAV microenvironment while increased cell survival was observed post-FLNA over-expression in an IAV microenvironment. (A) HEK293 cells were transfected with either pcDNA3.1 empty control vector or NP (PR8) plasmid in a dose-dependent manner. MTT assay was performed 24 h post-transfection. Results are shown as mean ± S.D. of one independent experiment (n = 5). Statistical significance was determined using one-way ANOVA with post-hoc Tukey test (∗p < 0.05). (B) HEK293 cells were transfected with pEGFP-N1 control plasmid (0.24 μg) or FLNA-GFP plasmid (0.24 μg) followed by IAV PR8 infection (MOI = 5) 24 h post-transfection. MTT assay was performed 24 h.p.i. Results are shown as mean ± S.D. of one independent experiment (n = 5). Statistical significance was determined using one-way ANOVA with post-hoc Tukey test (∗p < 0.05). (C) A549 cells were transfected with non-targeting control siRNA (NT) (200 nM) or FLNA siRNA (200 nM) followed by IAV PR8 infection (MOI = 5) 24 h post-transfection. MTT assay was performed 24 h.p.i. Results are shown as mean ± S.D. of one independent experiment (n = 5). Statistical significance was determined using one-way ANOVA with post-hoc Tukey test (∗p < 0.05).