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. 2020 Sep 25;11:581867. doi: 10.3389/fmicb.2020.581867

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Copyright © 2020 Sharma, Batra, Stuchlik, Reed, Pohl, Chow, Sambhara and Lal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Host FLNA co-localizes with IAV PR8 NP. (A) Shown is the cellular distribution of FLNA and NP proteins in IAV PR8 infected (MOI = 1) A549 cells at respective timepoints (220.5× magnification). (B) Shown is the cellular distribution of FLNA and NP proteins in NP PR8-transfected HEK293 cells (3 μg plasmid transfection) at respective timepoints (220.5× magnification). NP and FLNA were detected using mouse anti-NP antibody and rabbit anti-FLNA antibody, respectively. Alexa flour 488 (green-FLNA) and 594 (red-NP) conjugated secondary antibody were used. ProLong Gold Antifade Mountant with DAPI was used to stain the nucleus (blue) and mount the coverslip.