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. 2020 Sep 25;11:585530. doi: 10.3389/fimmu.2020.585530

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Copyright © 2020 Albuquerque-Souza, Schulte, Chen, Hardt, Hasturk, Van Dyke, Holzhausen and Kantarci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of hPDLSCs cementogenic-osteogenic differentiation after 7-day treatment in the osteogenic medium under different experimental conditions. (A) Representative figures for each condition showing ARS calcified deposits. (B) quantification (in nM) of extracted ARS calcified deposits. (C) the concentration of alkaline phosphatase (ALP; in ng/mL) measured at supernatants. (D–G) relative gene expression. (mRNA) of cementogenic-osteogenic markers (Runx2, Osteocalcin/OCN, CEMP1, and CAP) evaluated by qPCR using GAPDH as endogenous control and the ΔΔCT method. (*) p < 0.05 vs control (non-stimulated); (§) p < 0.05 vs IL-1β treated group; (^Δ) p < 0.05 vs TNF-α treated group; and (^ø) p < 0.05 vs IL-1β +TNF-α treated group.