FIGURE 5.

Identification and quantification of protein phosphorylation in control and the presence of MaR1, TNF-α, and MaR1/TNF-α. For each of the four experimental conditions, the phosphoproteome data from three independent biological replicates were collected. All phosphopeptides with FDR-adjusted p-values of ≤ 0.05 (ANOVA) passed the threshold criteria for statistical significance. (A) Hierarchical cluster analysis of the differentially expressed phosphoproteins and log2-fold changes between treatments and control. (B) Principal component analysis (PCA) showing a loading plot that visualizes the clustering of all biological replicates from the respective experimental groups (top) and a score plot that illustrates the grouping of statistically significant phosphopeptides (bottom). (C) Illustration of the total number of serine, threonine, and tyrosine phosphosites that were detectable in each experimental condition in three independent biological replicates. (*) p < 0.05 vs control (non-stimulated).