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. 2020 Sep 25;8:572749. doi: 10.3389/fcell.2020.572749

FIGURE 1.

FIGURE 1

Aberrant expression of TJs and epithelial cell markers in nasal biopsy specimens of CRSwNP patients. Representative IF staining of ZO-1 (A,D), occludin (B,E), Cldn3 (C,F) with MUC5AC (A–C) and βIV-tubulin (D–F) in nasal biopsies from control subjects and CRSwNP patients. TFI level of ZO-1, occludin, Cldn3, and βIV-tubulin were decreased in CRSwNP (G–I,K, respectively) whereas MUC5AC TFI level was increased in CRSwNP patients (J). The mRNA levels of ZO-1, occludin, and Cldn3 were decreased in CRSwNP as compared to control subjects (L–N). There was no significant change in mRNA expression of Foxj1 between two groups (P). Despite higher protein expression of MUC5AC in CRSwNP, its mRNA level was lower in CRSwNP tissue (O). Similarly, protein expression levels of ZO-1, occludin and Cldn3 were lower, and MUC5AC was higher in CRSwNP patients by western blotting assay (Q–W), whereas, Foxj1 protein level was not change between CRSwNP and control subjects (S,X). The mRNA levels were quantified by RT-qPCR assays, relative expression of the target gene was normalized to 2– ΔCT with GAPDH. Statistical analysis was calculated using the Mann–Whitney U test. Data were presented as median with an interquartile range. Scale bar = 20 μm.