FIGURE 2.

Correlation of TJs with goblet and ciliated cell in nasal biospecimen. TFI level of MUC5AC was not correlated with ZO-1 (A) but was negatively correlated with occludin and Cldn3 (B,C). βIV-tubulin TFI level was positively correlated with ZO-1, occludin, and Cldn3 (D–F) in CRSwNP and control subjects. IF staining of single primary nasal cells showed that both ZO-1 (G,J) and occludin (H,K) were co-stained with goblet and ciliated cell. Interestingly, Cldn3 positive staining was only observed in ciliated cell (L), but not goblet cell (I). Spearman r characteristic was performed for statistical analysis. Black dot refers to CRSwNP (n = 40) and red dot refers to control subjects (n = 20). Scale bar = 10 μm.