Skip to main content
. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581

FIGURE 1.

FIGURE 1

Efficient base editing with epi-ABEmax and epi-AncBE4max. (A) Schematic of the epi-ABEmax and epi-AncBE4max plasmids. The vector contains a mouse U6 promoter-driven gRNA scaffold, an EF1a promoter-driven base editor, an SV40 promoter-driven a blasticidin S deaminase (BSD), and oriP/EBNA1 elements for the plasmid replication in eukaryotes. (B) Procedure of base editing with epi-ABEmax and epi-AncBE4max. (C–F) Editing efficiency of epi-ABEmax in HEK293T, HeLa, H9, and iPSCs was measured at three-time points, n = 3 independent experiments. (G–J) Editing efficiency of epi-AncBE4max in HEK293T, HeLa, H9, and iPSCs was measured at three-time points, n = 3 independent experiments.