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. 2020 Sep 25;11:584254. doi: 10.3389/fimmu.2020.584254

FIGURE 2.

FIGURE 2

Pristane-induced inflammation in peripheral blood. WT mice were treated with or without pristane 14 days prior to analysis. (A) Representative flow cytometric analysis of Ly6C+ monocytes and Ly6G+ neutrophils gated on live cells. Numbers on plots indicate percentage within drawn gates. (B) Quantification of neutrophils and monocytes gated in (A). (C) Quantification of B220+ B cells and TCRβ+ T cells. (A–C) Cells are gated on Zombie negative live cells. (D,E) Serum cytokine levels measured by multiplex array. Representative of 3 independent experiments with 5 mice per group. *p < 0.05, **p < 0.01, by student’s t-test (B,C) or Mann-Whitney U test (D,E).