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. 2020 Sep 25;11:584254. doi: 10.3389/fimmu.2020.584254

FIGURE 4.

FIGURE 4

Abrogated ISG expression in pristane-treated IFNAR1– /– and IRF3/7– /– mice. WT, IFNAR1– /–, and IRF3/7– /– mice were treated with pristane 14 days prior to analysis. Untreated WT mice were included as controls. (A,B) Serum IFNα and CXCL10 levels measured by multiplex array. (C) Representative flow cytometric plots of CD11b+ F4/80int inflammatory macrophages, gated on live non-lymphocytes (Zombie– TCRβ– B220–) cells. Numbers on plots indicate percent of cells within drawn gates. (D) Histogram overlays and (E) quantification of Siglec-1 expression by inflammatory macrophages gated in (C). (F) Relative expression of Mx1, ISG15, and IRF7, compared to GAPDH, by all peritoneal fluid cells, measured by quantitative real-time PCR. Representative of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 by Kruskal-Wallis test with a Dunn’s post-test.