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. 2020 Sep 25;11:529614. doi: 10.3389/fimmu.2020.529614

Figure 2.

Figure 2

Conditioned medium of CS-Tat Jurkat cells augments CCL2 secretion from HUVEC. The cell-free conditioned media from the Jurkat cells (CS-Tat or CC-Tat or EV) were collected following 12 (left panel) or 36 h (right panel) of Dox induction. The conditioned media were added to HUVEC monolayers, and CCL2 secretion from the HUVEC was determined after 12 (top panel) or 24 h (bottom panel) of exposure to the conditioned media. The SM (HUVEC treated with RPMI supplemented with 10% FBS) served as the control for background CCL2 expression in the assay. The CCL2 levels were evaluated using an antigen-capture ELISA. EV, empty vector control; SM, serum medium control. The data are representative of three independent experiments. One-way ANOVA with Tukey–Kramer post-test (*p < 0.05, **p < 0.01, and ***p < 0.001).