Figure 3.

Tat neutralization in the conditioned media attenuates S31-specific differential CCL2 secretion. Conditioned media were collected from Jurkat-Tat cells at 12 h following Dox induction. The conditioned media were incubated with a highly potent anti-Tat monoclonal antibody (1 μg/ml of E2.1 antibody, raised in-house) or an IgG1 isotype control antibody or “no antibody” for 30 min at room temperature with gentle agitation. The HUVEC were exposed to the Tat-neutralized conditioned media for 12 h, and the CCL2 secretion was quantified using ELISA. EV: empty vector control. Two-way ANOVA with Bonferroni post-test (^***p < 0.001).