Table 2 |.

Conversion of a range of substrates using NMN⁺ as the cycling cofactor

Substrate	Product	Enzyme	Substrate concentrationa	Conversion (%)
		XenA	33 mM	>99
		XenA	10 mM	76 ± 2
		XenA	50 mM	49 ± 3
		OYE3	5 mM	>99
		NfsB	2 mM	92 ± 1
Cytochrome c (oxidized)	Cytochrome c (reduced)	P450 BM3 W1046S	50 μM	>99

Reactions were preformed in 200 mM potassium phosphate buffer (pH 7.5), 1 M NaCl, 300 mM d-glucose, 6 mM NMN⁺, and substrates at 30 °C while mixing for 24 h. Bs GDH was supplied at 0.33 mg ml⁻¹. XenA, OYE3, NsfB, and P450 BM3 W1046S were supplied at 0.75 mg ml⁻¹. Values are an average of at least three replicates with values after ± represent one standard deviation.

^aSubstrate concentration was limited by substrate solubility in the buffer.