Fig 4. Effect of high-speed spin and freeze/thaw on GLDH levels after APAP administration.

Plasma from APAP-dosed mice was isolated with only a standard low-speed spin (white) or with the addition of a high-speed spin (gray). GLDH was quantified in “fresh,” unfrozen samples (triangles) and again in samples that had undergone three freeze/thaw cycles (squares). Some fresh samples froze during transit and could not be measured, resulting in missing symbols. Each tick on the X-axis represents an individual APAP-dosed mouse and each mouse’s samples are separated by a vertical dotted line.