Fig 5. Effect of high-speed spin and freeze/thaw on GLDH levels after FS administration.

Plasma from FS-dosed mice was isolated with only a standard low-speed spin (white) or with a subsequent high-speed spin (gray). GLDH was quantified in “fresh,” unfrozen samples (triangles) and again in samples that had undergone three freeze/thaw cycles (squares). Some fresh samples froze during transit and could not be measured, resulting in absent symbols. Each tick on the X-axis represents an individual FS-treated mouse and each mouse’s samples are separated by a vertical dotted line.