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. 2020 Oct 9;15(10):e0239893. doi: 10.1371/journal.pone.0239893

Fig 4. Jhamt knockdown upregulates expression of ecdysteroid biosynthesis genes at 24 hao.

Fig 4

(A) Predicted ecdysteroid biosynthesis pathway in Daphnia magna. C: cholesterol; 7-dC: 7-dehydrocholesterol; 5-KD: 5β-ketodiol; 5-KT: 5β-ketotriol; 2dE: 2-deoxyecdysone; E: ecdysone; 20E: 20-hydroxyecdysone. Nvd: Neverland; Spo: Spook; Phm: Phantom; Dib: Disembodied; Sad: Shadow; Shd: Shade. (B) Expression of Nvd2, Spo, Dib, and Cyp18a1 at 24 h in siControl, siJhamt and siMet embryos at 24 h after siRNAt as measured by qRT-PCR. (C) Expression levels of other ecdysteroidogenesis genes in siControl, siJhamt, and siMet embryos. Expression levels are normalized to those of ribosomal L32, then shown relative to expression levels of siRNA-ctrl. Values are means. Error bars represent SD (N = 3). *p<0.05; **p<0.01; ***p<0.001 (Student’s t-test).