Skip to main content
. 2020 Sep 29;14(9):e0008590. doi: 10.1371/journal.pntd.0008590

Fig 3. B. pseudomallei K96243 type II and A4 are defective in MNGC formation in A549 and HeLa cells.

Fig 3

A549 cells (A) and HeLa cells (E) were infected with B. pseudomallei K96243 type I (B and F) and K96243 type II (C and G) and A4 (D and H) at MOI of 50 at 37°C for 12 h. The cells were stained with Giemsa stain. Percent MNGC formation in A549 cells (I) and HeLa cells (J) were quantified by number of nuclei in MNGCs x 100/total number of nuclei. The average size of MNGC in A549 (K) and HeLa cells (L) are shown as means ± standard deviation. Average MNGC size was calculated by total number of nuclei in MNGCs/total number of MNGCs. All assays were performed on two independent experiments in triplicate. Student’s t-test was performed to test the differences between bacterial strains. Scale Bars, 50 μm.