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. 2020 Oct 8;11(10):835. doi: 10.1038/s41419-020-03033-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The representative bar chart for the results of the qPCR analysis showed the increased expression of NPBWR1 in rat primary neurons transduced with Ad-Tat (MOI: 2, 72 h). b Immunoblotting showed an increase in NPBWR1 protein upon Tat expression. c qPCR showed upregulation of NPBWR1 in lncRNA-U1 induced primary neurons, independent of Tat expression. qPCR analysis indicated a 2-fold increased expression of NPBWR1 when primary neurons were transduced with Adeno-U1 (MOI:2) for 72 h. d Immunoblotting confirmed the overexpression of NPBWR1 upon lncRNA-U1 up-regulation in primary neurons. e ICC confirmed increased NPBWR1 expression in primary neurons upon Tat/U1 treatment, the result of immunoblotting revealed the formation of protein aggregates in the cell borders upon manipulating lncRNA-U1 expression in neurons. f q-PCR analysis compared the NPBWR1 expression in neurons transduced with Ad-U1, Ad-U1₆₆, Ad-U1R as well as Tat and co-transduction of Tat and U1R revealing a correlation between lncRNA-U1 overexpression and changes in NPBWR1 expression. U1₆₆ splice variant did not, however, alter NPBWR1 expression. g immunoblotting analysis showed no lncRNA-U1 minor splice variant and confirmed the results from q-PCR analysis.