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. 2020 Oct 8;10:16778. doi: 10.1038/s41598-020-73440-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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K. pneumoniae plasmid gene content. An UPGMA clustering was performed based on the plasmid DNA sequence for the determination of the genetic relation among the 22 plasmids. Similarity is indicated on the y-axis using a scale from 0 (not similar) to 100% (identical). A similarity of ≥ 85 to 100% is regarded as the same plasmid. The plasmids are indicated on the x-axis. The presence (black squares) and absence is indicated of annotated genes among the 22 plasmids of seven TGS sequenced isolates. If a gene was present twice, blue squares were used and more than 2, red squares were used. Colors indicated different groups of genes with a specific function. In the UPGMA tree, large plasmids are indicated in red, medium plasmids in black and small plasmids in green color.