Figure 5.

COI sequence alignments showing priming sites for both forward and reverse primers. Conserved regions to which universal primers bind are highlighted with blue squares. Positions at which nucleotides are the same as in universal primers are highlighted with pink texts in the primer sequences. COI sequences are highly divergent among amphipods whereas COI sequences from Torix Rickettsia from diverse host groups are highly conserved, which allowed the blocking primers (binding regions are highlighted with pink squares).