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. 2020 Oct 9;6(41):eabc7120. doi: 10.1126/sciadv.abc7120

Fig. 5. Dietary thiamine intake influences ASNase sensitivity of SLC19A2-low leukemia cells in vivo.

Fig. 5

(A) Plasma thiamine profiling of mice on conventional chow (n = 3 mice), and mice on a modified AIN-93G purified diet of low thiamine content (n = 8 mice) (mean ± SD). Human serum was profiled simultaneously for relative comparison. Statistics: *P < 0.01 by two-tailed unpaired t test for unequal variances. n.s., not significant. (B) Weights of mice initially on conventional chow, then switched to standard AIN-3G purified diet for 1 week, and lastly switched to a modified AIN-93G purified diet of low thiamine content (n = 8 mice) for indicated times, relative to initial weights on chow (mean ± SD). (C) Left: Kaplan-Meier survival curves of NSG mice on high- or low-thiamine AIN-93G diets engrafted with REH (endogenously low SLC19A2 cell line) by tail vein injection and treated with vehicle or ASNase (1000 U/kg, twice per week). Right: Box-and-whisker plots of survival data. Statistics: Left: n.s., Mantel-Cox P > 0.05/3 (Bonferroni correction); right: n.s., two-tailed unpaired t test for equal variances P > 0.05/3 (Bonferroni correction). For both analyses, **P < 0.005. n = 5 mice for untreated groups and n = 7 mice for ASNase groups. (D) Schematic depicting that environmental thiamine influences ASNase sensitivity of leukemia cells with low SLC19A2 expression.