Skip to main content
. 2020 Oct 6;11(40):3633–3645. doi: 10.18632/oncotarget.27702

Figure 5. miR-210 silences the proapoptosis member CASP8AP2.

Figure 5

Western blot of CASP8AP2 in (A) A673 and SK-ES-1 Spheres transfected with either miR-scramble or miR-210 inhibitors under normoxic and hypoxic conditions. (B) A673 and SK-ES-1 spheres transfected with miR-scramble, miR-210 mimics, miR-210 inhibitors or 20 μg/ml hypoxic exosomes. (C) A673 and SK-ES-1 spheres transfected with Non-Targeting control (NTC) or CASP8AP2-siRNA. α-Tubulin was used as the internal loading control. (D) Sphere assay quantifying A673 and SK-ES-1 cells transfected with either NTC or CASP8AP2-siRNA under normoxic and hypoxic conditions. (Mean + SEM, n = 3, * P ≤ 0.002, ** P ≤ 0.0002, *** P ≤ 0.0001). (E) Assessment of apoptosis by Annexin V-FITC/PI staining. (Left) Representative plots and (Right) Quantification of Annexin V-FITC stainined A673 and SK-ES-1 spheres transfected with NTC or CASP8AP2-siRNA under normoxic and hypoxic conditions. (Mean + SEM, n = 3, * P ≤ 0.0008, ** P ≤ 0.0001).