Table 1.
Staining panel including CD16 among myeloid markers, grouping all DC markers in APC.
Antibody target | Available source | Catalog # | Clone | Fluorophore | |
---|---|---|---|---|---|
1 | Anti-CD3 | Biolegend | 317329 | OKT3 | BV785 |
2 | Anti-CD45 | Biolegend | 304023 | HI30 | Alexa Fluor 700 |
3 | Anti-CD11b | Biolegend | 101217 | M1/70 | Alexa Fluor 488 |
4 | Anti-CD68 | Biolegend | 333821 | Y1/82A | APC-Cy7 |
5 | Anti-CD32 | Biolegend | 303205 | FUN-2 | PE |
6 | Anti-CD14 | Biolegend | 367111 | 63D3 | PE-Cy7 |
7 | Anti-CD206 | Biolegend | 321121 | 15-2 | PerCP-Cy5.5 |
8 | Anti-CD16 | eBioscience | 48-0168-42 | eBioCB16 (CB16) | eFluor 450 (BV421) |
9 | Anti-CD1c + Anti-CD303 + Anti-CD141 | all Miltenyi | 130-110-595 + 130-113-752 + 130-113-876 | REA694 + AC144 + AD5-14H12 | all APC |
10 | Live/Dead staining | Thermo-Fisher | L23105 | n/a | UV-DAPI |