Table 2.
Staining panel including CD206 and the three classic human DC markers, BDCA-1 (CD1c), BDCA-1 (CD303), and BDCA-3 (CD141).
| Antibody target | Source | Catalog # | Clone | Fluorophore | |
|---|---|---|---|---|---|
| 1 | Anti-CD3 | Biolegend | 317329 | OKT3 | BV785 |
| 2 | Anti-CD45 | Biolegend | 304023 | HI30 | Alexa Fluor 700 |
| 3 | Anti-CD11b | Biolegend | 101217 | M1/70 | Alexa Fluor 488 |
| 4 | Anti-CD68 | Biolegend | 333821 | Y1/82A | APC-Cy7 |
| 5 | Anti-CD14 | Biolegend | 367111 | 63D3 | PE-Cy7 |
| 6 | Anti-CD206 | Biolegend | 321121 | 15-2 | PerCP-Cy5.5 |
| 7 | Anti-CD1c (BDCA-1) | Miltenyi | 130-110-594 | REA694 | PE |
| 8 | Anti-CD303 (BDCA-2) | Miltenyi | 130-106-505 | AC144 | VioBlue (BV421) |
| 9 | Anti-CD141 (BDCA-3) | Miltenyi | 130-113-876 | AD5-14H12 | APC |
| 10 | Live/Dead staining | Thermo-Fisher | L23105 | n/a | UV-DAPI |