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. 2020 Sep 24;11:543148. doi: 10.3389/fphys.2020.543148

Figure 5.

Figure 5

Effect of GLUT1 on embryo implantation in mice. (A) Adhesion of JAR cells onto RL95-2 endometrial epithelial cells was observed in response to GLUT1 silencing in vitro (scale bar = 100 μm). (B) The relative adhesion ratio of JAR cells on endometrial cells was analyzed by Image-Pro Plus software (n = 3). (C) Western blot analysis of GLUT1 in RL95-2 cells treated with NC siRNA or GLUT1 siRNA. (D) Typical photograph showing mice uteri on D7 of mating after injecting mus-NC siRNA (left) or GLUT1 siRNA (right). (E) The implanted embryos ratio was analyzed after injecting NC siRNA or GLUT1 siRNA (n = 5). (F) Western blot analysis of GLUT1 and G6PD in mice uteri on D7 of mating after injecting mus-NC siRNA (left) or GLUT1 siRNA (right). (G) Relative density analysis of the GLUT1 and G6PD by Image Lab 4.0 software (n = 3). (H) The qPCR analysis of Glut1 mRNA levels of embryos in the uterus of the transfection group and the NC group (n = 3, normalized by Gapdh, ns, no significant difference). Error bars represent the mean ± SD. Student’s t-test, * p < 0.05, ** p < 0.01.