TABLE 1.
Primers and probes used in this study.
|
PCR P. aeruginosa AUS531 mutant (AUS531Δbci) | |||
| Primer | Sequence (5′-3′) | Restriction Site | Reference |
| UP_Bci Fow | GGGGGTACCGCACCGCAACCTCCCGATCA | KpnI | This study |
| UP_Bci Rev | GGGCTCGAGGGCGTAACTCCGTTCGAGGG | XhoI | This study |
| DOWN_Bci Fow | GGGCTCGAGCGCCTGGCCTATTGCCGGGC | XhoI | This study |
| DOWN_Bci Rev | GGGGGATCCGTCGTCGATGATTGAGCGAA | BamHI | This study |
| INT UP Fow | ATTGTAGTCATACTCAAGAC | – | This study |
| INT DOWN Rev | TGCACCGCCTTATGTGAAAG | – | This study |
| pEX18 Fow | GGCTCGTATGTTGTGTGGAATTGTG | – | This study |
| pEX18 Rev | GGATGTGCTGCAAGGCGATTAAG | – | This study |
|
RT-qPCR P. aeruginosa AUS531 mutant (AUS531Δbci) | |||
| Primer | Sequence (5′-3′) | UPL probea | References |
| proC_149_Fw | CTGTCCAGCGAGGTCGAG | 149 | Tan et al., 2015 |
| proC_149_Rev | CCTGCTCCACCAGTGCTT | ||
| LasR_139_Fw | GATATCGGTTATCTGCAACTGCT | 139 | This study |
| LasR_139_Rev | CCGCCGAATATTTCCCATA | ||
| RhlR_115_Fw | TGCGTTGCATGATCGAGT | 115 | This study |
| RhlR_115_Rev | CGGGTTGGACATCAGCAT | ||
| QscR_133_Fw | GTTCCAGCGAGAGCATCG | 133 | This study |
| QscR_133_Rev | TGGTGATCCAGAGCAGGAA | ||
| PqsR_151_Fw | TCGACACCAAGGTGTATTGC | 151 | This study |
| PqsR_151_Rev | TCGAGAAAGCGCAGGAAG | ||
aUniversal Probe Library (UPL) (Roche, Germany; https://lifescience.roche.com/en_es/brands/universal-probe-library.html).