Table 3.

Transgene-free BC1 mutants obtained from crossing each of the primary mutants with genetically compatible wild-type plants.

T0 Mutant	# Progeny analyzed†	# of Cas9 negative‡	Cas9 negative
			Monoallelic Pvtb1a-Pvtb1b mutant	Monoallelic Pvtb1b mutant	Monoallelic Pvtb1a mutant	Nonmutant
52-1	30	12	5	5	0	2
35-2	20	18	0	0	2	16
35-1	6	3	1	0	0	2

^†BC1 seeds collected from each of the primary mutants were germinated and allowed to grow for a month.

^‡The presence of the transgene was analyzed using PCR with gRNA/Cas9-specific primers (Supplementary Table S6).