Table 4.

The average tiller number of transgene-free BC1 mutants and that of the siblings carrying no mutations (null segregants) and the percentage increase of tiller number for mutants over nonmutant siblings.

Genotype	N	Average tiller number	% increase of tiller number in mutant over the WT
AABb (mutant)	4	26.0 ± 4.7b	30 ± 23
AaBb (mutant)	5	29.2 ± 4.4b	45 ± 20
AABB (WT)	6	20.0 ± 1.4a	0 ± 10

All data are shown as mean ± standard deviation. A and a represent the wild-type and mutant allele of Pvtb1a gene, respectively whereas B and b represent the wild-type and the mutant allele of the Pvtb1b gene, respectively. The percentage increase of tiller number for each genotype was calculated by comparing the mutant plants with the wild-type plants at the same developmental stage using the one-tailed Student’s t-test. Different letters at the same column indicate significant differences at P < 0.05 level.