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. 2020 Sep 25;11:532199. doi: 10.3389/fphar.2020.532199

Figure 1.

Figure 1

Immunization protocol with Rv3619c/IFA in ovalbumin (OVA)-challenged murine model of asthma. 6–8 weeks ok female balb/c mice (n = 10) were immunized intraperitonealy on day 0 with either 2 µg of Rv3619c emulsified with Freund’s Incomplete Adjuvant (IFA) and boosted with protein only on days 14 and 28 [Rv3619c/IFA and Rv3619c/IFA+ dexamethasone (DEX) (0.5 mg) groups]. All other groups (n = 10) were immunized with PBS emulsified with IFA on day 0 and PBS only on days 14 and 28. All mice (except PBS group mice) were immunized intraperitoneally with OVA/AluG on day 42. Mice were then challenged intranasally with OVA for four consecutive days (days 52 to 55). The PBS group mice were challeneged intranasally with PBS on the same days. Mice in Rv3619c/IFA/dexamethasone (DEX) (0.5 mg), dexamethasone (DEX) (0.5 mg), and dexamethasone (DEX) (3 mg) groups were treated with either dexamethasone an hour prior to intranasal challenge. All mice were sacrificed on day 56 and Bronchoalveolar lavage fluid (BALF) lung tissue, blood and spleens were collected.