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. 2020 Sep 25;14:546659. doi: 10.3389/fncel.2020.546659

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Copyright © 2020 Noh, Oh, Park and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Peri-infarct angiogenesis after IC transplantation or IV injection of hUC-MSCs in MCAo rats. (A) Representative confocal images of DAB-stained RECA-1 (brown) in four study groups. Scale bar = 100 μm. (B) Schematic images of quantitative analyses for four ROIs in peri-infarct border zones. (C) Quantitative analyses of RECA-1⁺ microvascular (diameter < 30 μm) counts in four study groups (n = 5 each). (D) Representative confocal images of dual IHC for PECAM1 (green) and BrdU (red) in the rat after IC transplantation of hUC-MSCs. Note: No PECAM1⁺/BrdU⁺ vessels were found in the other three study groups. DAPI (blue) counterstain. Scale bar = 50 μm. Data expressed as mean ± SEM values. *p < 0.05.