Skip to main content
. 2020 Sep 24;11:572634. doi: 10.3389/fimmu.2020.572634

FIGURE 2.

FIGURE 2

Proportion of donor plasma samples outside the assay quantification range for the Luminex (LMX) and Meso Scale Discovery (MSD) multiplex cytokine immunoassay kits. Although the MSD assay had a LLoQ below that of the LMX assay for 14/16 common analytes in earlier standard curve performance evaluations, this did not directly translate to evaluation of cytokines in experimental human plasma samples, whereby several analytes were missed at a greater frequency with the MSD platform (e.g., IL-10, IL-12, IL-1β, IL-2, IL-4, IL-6, and GM-CSF) and others were missed at a greater frequency with the LMX platform but detected with the MSD assay (e.g., IFN-γ, IL-5, IL-7, IL-17, MIP-1α, and MIP-1β).