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. 2020 Sep 25;7:545359. doi: 10.3389/fvets.2020.545359

Table 6.

Percentages of yolk, albumen, and eggshell of the eggs produced by laying hens fed rations containing different concentrations of caffeine for 12 weeks.

Caffeine in diet Experimental period
4 weeks 8 weeks 12 weeks
Percentage of yolk
0 (control) 28.1 ± 0.42 27.9 ± 0.41 27.4 ± 0.27a
150 ppm 28.0 ± 0.40 28.6 ± 0.35 27.6 ± 0.41a, b
300 ppm 28.4 ± 0.37 28.9 ± 0.45 28.4 ± 0.57b
450 ppm 28.2 ± 0.40 28.3 ± 0.30 28.9 ± 0.49b
p n.s. n.s. <0.05
Percentage of albumen
0 (control) 62.3 ± 0.45 62.5 ± 0.47 62.7 ± 0.32
150 ppm 62.6 ± 0.43 62.3 ± 0.44 63.7 ± 0.38
300 ppm 62.6 ± 0.40 62.1 ± 0.44 63.0 ± 0.63
450 ppm 62.9 ± 0.42 63.0 ± 0.34 62.4 ± 0.50
p n.s. n.s. n.s.
Percentage of eggshell
0 (control) 9.61 ± 0.14a 9.64 ± 0.11a 9.87 ± 0.14a
150 ppm 9.42 ± 0.11a 9.16 ± 0.14a, b 8.72 ± 0.18b
300 ppm 9.04 ± 0.12b 8.95 ± 0.10b 8.56 ± 0.20b
450 ppm 8.93 ± 0.14b 8.73 ± 0.15b 8.78 ± 0.17b
p <0.001 <0.001 <0.0001

Data are shown as mean ± SEM.

a, bDifferent letters in the same column show significant difference (p < 0.05, Kruskal-Wallis test followed by Student-Newman-Keuls test).

n.s., non-significant.

n = 24.