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. 2020 Oct 9;3:564. doi: 10.1038/s42003-020-01278-5

Fig. 2. H2N2 viral replication and disease manifestation are reduced by prior H1N1-priming.

Fig. 2

a Outline of the study. Ferrets were primed with H1N1 (A/California/07/2009) or received PBS on day 0. Subsequently, both primed and non-primed animals were infected with H2N2 (A/Singapore/1/57) on day 28. The placebo group received PBS on both occasions. All animals were euthanized on day 42 to analyze T cell responses. b Viral replication in throat and nose of H2N2-infected ferrets prior to infection (day 28) and 2, 4, and 7 days after infection (days 30, 32, and 35 respectively). Lines depict individual ferrets (n = 6). c Relative changes in weight from the moment of H2N2 infection until the end of the experiment (n = 6). Data are displayed as mean ± SD. d Temperature displayed as mean deviation from baseline in 30 min intervals from the day of H2N2 infection until day 38 (n = 5–6). Baseline temperature was calculated as the average temperature over 4 days prior to H2N2 infection. Asterisk indicates significant differences (p < 0.05) between non-primed and H1N1-primed groups after correction for multiple testing. a was created using Biorender.